Luria Bertani Broth, Miller 500Gm SRL

Applications:
Used for maintaining and propagating Escherichia coli in molecular microbiology procedures.

Composition:
Ingredients : gm/lt.
Tryptone : 10.00
Yeast extract : 5.00
Sodium chloride : 10.00

Directions:
1. Add 25.00 gm powder to distilled/deionized water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.